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Biology Lab Activities: Cellular Respiration

Background Questions

Based on what you’ve learned in class, in your textbook, and from using Visible Biology, answer the following questions about cellular respiration.

1. Fill in the blanks in the cellular respiration equation: C6H12O6 + 6 _______ → 6 CO2 + 6 ______ + up to 38 molecules of _______.

2. What are the reactants involved in cellular respiration?

3. What are the products of cellular respiration?

4. Cellular respiration consists of four main phases. Complete the following sentences about these phases and where they take place.

a. ______ takes place in the cytosol.

b. Pyruvate oxidation takes place in the ______.

c. The ______ cycle takes place in the mitochondrial matrix.

d. Oxidative phosphorylation takes place in the _______________.

5. In 2–3 sentences, explain why cellular respiration is important for a eukaryote’s survival.

Name: Date:

Lab 1: Mitochondria Structures, Reactants, and Products of Cellular Respiration

Activity 1: Label a mitochondrion

1. Launch the view

· Launch Visible Biology.

· Navigate to Study/Lab Activities, and find the Cellular Respiration Lab section.

· Select view 1. Mitochondrion (Cellular Respiration).

2. Label the image below

· Explore the 3D model of the mitochondrion going through cellular respiration to find the structures you need to label.

· Fill in the blanks to label the structures from the list below.

Part A: Label the mitochondria structures

Word List:

1

Cristae

Inner membrane

Matrix

Outer membrane

___

___

___

___

Part B: Label the reactants and products of glycolysis

Word List:

ATP

ADP

Glucose (C6H12O6) molecule

Pyruvates

___

___

___

___

Part C: Label the reactants and products of pyruvate oxidation and the citric acid cycle

Word List:

Acetyl-CoA

ATP

Carbon dioxide (CO2) molecules

___

___

___

Part D: Label the reactants and products of oxidative phosphorylation

Word List:

ATP

Oxygen (O2) molecules

Water (H2O) molecules

___

___

___

Name: Date:

Lab 1: Mitochondria Structures, Reactants, and Products of Cellular Respiration

Activity 2: Explore the roles mitochondria structures play in cellular respiration

Refer to your labeled mitochondrion model from Activity 1 and the content in Visible Biology. Based on what you’ve learned, match each of the following structures with the brief description of its role in cellular respiration.

Structures:

a. Inner membrane

b. Matrix

c. Cristae

d. Cytosol

Descriptions:

___ This structure contains the electron transport chain, and chemiosmosis occurs across this structure to produce water molecules and ATP.

___ These structures increase the surface area of the inner mitochondrial membrane to facilitate gas exchange.

___ This fluid surrounds the mitochondria, and it’s where glycolysis takes place.

___ This is the fluid-filled inner part of the mitochondrion, where the citric acid cycle takes place.

Name: Date:

Lab 1: Mitochondria Structures, Reactants, and Products of Cellular Respiration

Activity 3: Explore the reactants and products of cellular respiration

Refer to your labeled reactants and products of cellular respiration from Activity 1 and the content in Visible Biology. Based on what you’ve learned, complete the following table on the molecules involved in cellular respiration.

Based on what you’ve learned from labeling the mitochondrion (cellular respiration) model and completing the table, fill in the blanks to balance the cellular respiration equation:

C6H12O6 + 6 _______ → 6 ________ + 6 H2O + up to 38 molecules of _________.

Name: Date:

Lab 2: Cellular Respiration in the Mitochondrion

Refer to your labeled reactants and products of cellular respiration image from Lab 1, Activity 1.

1. In the space that follows, draw a mitochondrion (or paste a screenshot of your drawing). Your drawing should include the following:

a. Inside the mitochondrion, draw the inner and outer membranes, cristae, and matrix.

b. Outside the mitochondrion, draw the cytosol, as well as the ATP molecules, carbon dioxide (CO2) molecules, water (H2O) molecules, oxygen (O2) molecules, glucose (C6H12O6) molecules, NAD+, NADH, FAD, FADH2, and electrons with arrows showing how they move into, out of, or through the mitochondrion. Be sure to accurately portray the number of each of these molecules in your drawing.

2. Based on your drawing and what you’ve learned about cellular respiration, put the following steps of cellular respiration into the correct order (from 1–5).

___ Within the mitochondrion, the pyruvates are converted into acetyl-CoA.

___ Glucose is converted into fructose, which is converted into two pyruvates.

___ The electron transport chain and chemiosmosis produce water molecules and ATP.

___ Acetyl-CoA goes into the citric acid cycle, producing carbon dioxide, ATP, NADH, FADH2, and electrons.

___ NADH and FADH2 bring electrons to the electron transport chain.

3. In your own words, briefly describe the cellular respiration equation.

4. The equation for photosynthesis is 6CO2 + 6H2O → C6H12O2 + 6O2. Based on this equation and what you’ve learned about cellular respiration, how do these two processes relate to each other?