answer the question in excel following the order order The experiment found the expression of plant-Defence related

answer the question in excel following the order

order

The experiment found the expression of plant-Defence related genes in pathogenic infectious conditions and conditions in which beneficial microorganisms coexist with plants.
Treatment 1 neg Sterile tree
Treatment 2 Xcc The tree is infected with canker disease in lemon. (Xanthomonas citri pv citri)
Treatment 3 Pse A plant that has been planted with useful germs (Pseudomonas sp.)
Gene of Interest: Plant-defense related gene
LRRL leucine-rich repeat receptor-like protein gene
RPP13L Disease resistance protein RPP13 gene
CHSTL Chalcone synthase Like gene
BSK1L Brassinosteroid-signaling kinase 1 Like gene
House keeping gene
Ef1a gene (Elongation Factor 1 a Gene)
question Find the expression of Plant-defense related gene (choose 1 gene) method ?
Fold difference = 2^-ΔΔCt
Fill the available values in the table.
Create an easy-to-understand bar chart.
Ready to explain / write a summary
ANSWER Find average cq
Find the SD and consider the statistics to use
Find delta Ct
Find delta delta Ct
Find Fold difference = 2^-ΔΔCt

information

Sample 6 h Ef1a gene (House Keeping Gene) RPP13L gene ∆Ct ∆∆Ct 2^-∆∆Ct
replicates for each cq rep. 1 cq rep. 2 cq rep. 3 ave cq sd cq rep. 1 cq rep. 2 cq rep. 3 ave cq sd
1 neg 1 18.5 18.53 18.49 21.67 21.5 21.8
2 neg 2 20.34 20.45 20.4 23.3 23.6 23.7
3 neg 3 19.04 19.18 19.12 22.48 23.24 22.96
4 neg 4 20.13 20.05 20.06 22.73 22.8 22.65
5 neg 5 21.79 22.37 22.01 24.87 24.50 25.02
1 xcc 1 19.33 19.31 19.3 22.13 22.5 21.87
2 xcc 2 20.91 21.15 21.07 24.14 23.45 23.7
3 xcc 3 20.53 20.34 20.46 22.75 22.92 23.41
4 xcc 4 20.92 20.87 20.94 22.03 22.48 22.64
5 xcc 5 21.83 22.26 22.01 23.78 23.43 23.5
1 pse 1 20.92 20.69 20.73 22.43 22.82 22.23
2 pse 2 19.42 19.50 19.47 21.38 21.11 21.65
3 pse 3 20.60 20.61 20.58 22.17 22.56 22.49
4 pse 4 20.09 20.53 20.43 22.04 21.54 22.16
5 pse 5 23.31 23.43 23.35 25.76 25.40 24

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