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answer the question in excel following the order order The experiment found the expression of plant-Defence related

August 24, 2024

answer the question in excel following the order

order

	The experiment found the expression of plant-Defence related genes in pathogenic infectious conditions and conditions in which beneficial microorganisms coexist with plants.
Treatment 1	neg	Sterile tree
Treatment 2	Xcc	The tree is infected with canker disease in lemon. (Xanthomonas citri pv citri)
Treatment 3	Pse	A plant that has been planted with useful germs (Pseudomonas sp.)
Gene of Interest: Plant-defense related gene
	LRRL	leucine-rich repeat receptor-like protein gene
	RPP13L	Disease resistance protein RPP13 gene
	CHSTL	Chalcone synthase Like gene
	BSK1L	Brassinosteroid-signaling kinase 1 Like gene
House keeping gene
	Ef1a gene (Elongation Factor 1 a Gene)
question	Find the expression of Plant-defense related gene (choose 1 gene) method ?
	Fold difference = 2^-ΔΔCt
	Fill the available values in the table.
	Create an easy-to-understand bar chart.
	Ready to explain / write a summary
ANSWER	Find average cq
	Find the SD and consider the statistics to use
	Find delta Ct
	Find delta delta Ct
	Find Fold difference = 2^-ΔΔCt

information

	Sample 6 h	Ef1a gene (House Keeping Gene)					RPP13L gene					∆Ct	∆∆Ct	2^-∆∆Ct
replicates for each		cq rep. 1	cq rep. 2	cq rep. 3	ave cq	sd	cq rep. 1	cq rep. 2	cq rep. 3	ave cq	sd
1	neg 1	18.5	18.53	18.49			21.67	21.5	21.8
2	neg 2	20.34	20.45	20.4			23.3	23.6	23.7
3	neg 3	19.04	19.18	19.12			22.48	23.24	22.96
4	neg 4	20.13	20.05	20.06			22.73	22.8	22.65
5	neg 5	21.79	22.37	22.01			24.87	24.50	25.02
1	xcc 1	19.33	19.31	19.3			22.13	22.5	21.87
2	xcc 2	20.91	21.15	21.07			24.14	23.45	23.7
3	xcc 3	20.53	20.34	20.46			22.75	22.92	23.41
4	xcc 4	20.92	20.87	20.94			22.03	22.48	22.64
5	xcc 5	21.83	22.26	22.01			23.78	23.43	23.5
1	pse 1	20.92	20.69	20.73			22.43	22.82	22.23
2	pse 2	19.42	19.50	19.47			21.38	21.11	21.65
3	pse 3	20.60	20.61	20.58			22.17	22.56	22.49
4	pse 4	20.09	20.53	20.43			22.04	21.54	22.16
5	pse 5	23.31	23.43	23.35			25.76	25.40	24

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